The studs will hold the gasket in place, and will probably not allow any movement of the head. Especially if the nuts and washers are centering. If you can move the head side to side at all when the nuts are not quite finger tight, yes, you should. If the head is solid, and isn't going anywhere at all, then no, you don't. Since there weren't any when you removed the heads, I suspect the heads will have no play at all when the nuts and washers are finger tight.
Take care,
~Chris
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